{"id":12461,"date":"2024-11-25T12:06:54","date_gmt":"2024-11-25T11:06:54","guid":{"rendered":"https:\/\/www.gba-polska.pl\/cosmetics\/badania-in-vitro\/"},"modified":"2025-01-23T13:19:59","modified_gmt":"2025-01-23T12:19:59","slug":"in-vitro_tests","status":"publish","type":"page","link":"https:\/\/www.gba-polska.pl\/en\/cosmetics\/in-vitro_tests\/","title":{"rendered":"In vitro tests"},"content":{"rendered":"<p><section id=\"bt_bb_section69e49d5380e4b\" class=\"bt_bb_section bt_bb_color_scheme_11 bt_bb_layout_boxed_1200 bt_bb_vertical_align_top bt_bb_background_overlay_dark_solid bt_bb_top_spacing_extra_large bt_bb_bottom_spacing_large bt-dark-skin\" style=\"; --section-primary-color:#ffffff; --section-secondary-color:#181818;\" data-bt-override-class=\"null\"><div class=\"bt_bb_background_image_holder_wrapper\"><div class=\"bt_bb_background_image_holder btLazyLoadBackground\"  data-background_image_src=\"https:\/\/www.gba-polska.pl\/wp-content\/uploads\/2021\/12\/20211214_GBA_Polska_Slider_v3-1.jpg\" data-parallax-zoom-start=\"1\" data-parallax-zoom-end=\"1\" data-parallax-blur-start=\"0\" data-parallax-blur-end=\"0\" data-parallax-opacity-start=\"1\" data-parallax-opacity-end=\"1\" style=\" background-image: url(&#039;https:\/\/www.gba-polska.pl\/wp-content\/plugins\/bold-page-builder\/img\/blank.gif&#039;);\"><\/div><\/div><div class=\"bt_bb_port\"><div class=\"bt_bb_cell\"><div class=\"bt_bb_cell_inner\"><div class=\"bt_bb_row \"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div  class=\"bt_bb_column col-xxl-6 col-xl-6 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal bt_bb_animation_fade_in zoom_out animate\" style=\"; --column-width:6;\" data-width=\"6\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><header class=\"bt_bb_headline bt_bb_dash_top bt_bb_superheadline bt_bb_size_large bt_bb_align_inherit\" data-bt-override-class=\"null\"><h1><span class=\"bt_bb_headline_superheadline\"><span>Cosmetics testing<\/span><\/span><span class=\"bt_bb_headline_content\"><span>In vitro tests<span class=\"bt_bb_outline_text bt_bb_outline_text_align_left bt_bb_outline_text_size_400\"><span>Badania<\/span><\/span><\/span><\/span><\/h1><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_60\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-6 col-xl-6 bt_bb_vertical_align_top bt_bb_align_right bt_bb_padding_40 bt_bb_animation_fade_in zoom_out animate\" style=\"; --column-width:6;\" data-width=\"6\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><\/div><\/div><\/div><\/div><\/div><\/div><!-- cell_inner --><\/div><!-- cell --><\/div><!-- port --><\/section><section id=\"bt_bb_section69e49d5381665\" class=\"bt_bb_section bt_bb_layout_wide bt_bb_vertical_align_top bt_bb_top_spacing_large bt_bb_bottom_spacing_large\" style=\"z-index: 1;\" data-bt-override-class=\"null\"><div class=\"bt_bb_port\"><div class=\"bt_bb_cell\"><div class=\"bt_bb_cell_inner\"><div class=\"bt_bb_row bt_bb_row_push_left bt_bb_content_wide bt_bb_row_width_boxed_1200 bt_bb_row_width_boxed  bt_bb_column_gap_30\" style=\"; --column-gap:30px;\" data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div  class=\"bt_bb_column col-xxl-5 col-xl-5 bt_bb_vertical_align_bottom bt_bb_align_left bt_bb_padding_normal bt_bb_animation_fade_in animate bt_bb_hidden_xs bt_bb_hidden_ms bt_bb_hidden_sm\" style=\"position: relative;; --column-width:5;\" data-width=\"5\" data-bt-override-class=\"{&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_left&quot;,&quot;def&quot;:&quot;left&quot;,&quot;xl&quot;:&quot;left&quot;}}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div class=\"bt_bb_image bt_bb_shape_square bt_bb_align_inherit bt_bb_hover_style_simple bt_bb_content_display_always bt_bb_content_align_bottom bt_bb_remove_padding_40 bt_bb_fill_background_color_match_content_height\" style=\"max-width: 650px; float: right;\" data-bt-override-class=\"null\"><span><img decoding=\"async\" src=\"https:\/\/www.gba-polska.pl\/wp-content\/uploads\/2024\/12\/GBA-POLSKA-in-vitro-tests.jpg\" data-full_image_src=\"https:\/\/www.gba-polska.pl\/wp-content\/uploads\/2024\/12\/GBA-POLSKA-in-vitro-tests.jpg\"  title=\"GBA-POLSKA-in-vitro-tests\" alt=\"https:\/\/www.gba-polska.pl\/wp-content\/uploads\/2024\/12\/GBA-POLSKA-in-vitro-tests.jpg\"><\/span><\/div><div class=\"bt_bb_floating_element bt_bb_floating_element_horizontal_position_right bt_bb_floating_element_vertical_position_top bt_bb_floating_element_animation_delay_default bt_bb_floating_element_animation_duration_default bt_bb_floating_element_animation_style_ease_out\" style=\"margin-right: -1em; margin-top: -3em; mix-blend-mode: multiply;\" data-speed=\"0\"><div class=\"bt_bb_floating_element_html\" data-speed=\"0\"><span  style=\"width: 6em; height: 6em; box-shadow: 0 0 0 4px #F1F1F1 inset;\"><\/span><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_60 bt_bb_hidden_md bt_bb_hidden_lg\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-7 col-xl-7 bt_bb_vertical_align_middle bt_bb_align_left bt_bb_padding_normal bt_bb_animation_fade_in animate\" style=\"; --column-width:7;\" data-width=\"7\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div class=\" bt_bb_row_inner\"   data-bt-override-class=\"{}\"><div class=\"bt_bb_row_inner_holder\"  ><div  class=\"bt_bb_column_inner col-xxl-12 col-xl-12 col-xs-12 col-sm-12 col-md-12 col-lg-12 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal\"  data-width=\"12\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_inner_content\"><div class=\"bt_bb_column_inner_content_inner\"><div  class=\"bt_bb_text\" ><\/p>\n<p>In the GBA Polska In Vitro Lab we offer the complete spectrum of assays to discover new biological efficacies of cosmetic ingredients.<\/p>\n<p>We offer:<\/p>\n<ul>\n<li><strong>Enzymatic tests<\/strong>.<\/li>\n<li>Evaluation of the <strong>gene expression:<\/strong> Measurement of the level of expression of genes linked with the biological processes of interest in skin, such as collagen and elastin production, skin hydration, melanin production, skin biomechanic properties, skin ageing processes, cells metabolism, cells renewal etc. This type of research is a first step in evaluation of the substance efficacy.<\/li>\n<li>Evaluation of the <strong>production of proteins<\/strong> responsible for the skin structure and the correct biological processes in different types of cells.<\/li>\n<li>Evaluation of the <strong>skin permeation<\/strong>.<\/li>\n<\/ul>\n<p><strong>Our experimental models: <\/strong><\/p>\n<ul>\n<li>We run our studies on different types of cells: fibroblasts NHDF, melanocytes NHM, keratinocytes NHEK and HaCaT, human cells of hair follicle papilla, and other.<\/li>\n<li>We run studies on bacterial strains characteristic for some common skin problems, ex. <em>Cutibacterium acnes<\/em>.<\/li>\n<li>We offer Epidermis 3D (Reconstructed Epidermis 3D) and cells co-cultures models.<\/li>\n<li>We use a simplified skin microbiome model.<\/li>\n<li>We offer the possibility of personalizing the design of the experimental model and the experimental set-up according to our clients\u2019 needs.<\/li>\n<li>We offer the possibility of applying the stress conditions to the experimental set-up, ex. air pollution, UV, IR, oxidative stress.<\/li>\n<\/ul>\n<p>\n<\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_30 bt_bb_hidden_md bt_bb_hidden_lg\" data-bt-override-class=\"null\"><\/div><header class=\"bt_bb_headline bt_bb_dash_top bt_bb_size_small bt_bb_align_left\" data-bt-override-class=\"null\"><h3 style=\"font-size:28px\"><span class=\"bt_bb_headline_content\"><span>IN VITRO EVALUATION OF THE EFFICACY \/ BIOACTIVITY OF COSMETIC INGREDIENTS<\/p><br \/>\n<p>CLAIMS AND SUPPORTING IN VITRO TESTS:<\/span><\/span><\/h3><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_30 bt_bb_hidden_md bt_bb_hidden_lg\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_accordion bt_bb_color_scheme_5 bt_bb_style_simple bt_bb_shape_square\" style=\"; --accordion-primary-color:#000000; --accordion-secondary-color:#004787;\"><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">1. CLAIM: Skin color<\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<ul>\n<li><strong>Skin tone<\/strong>: Skin whitening, Skin lightening, Skin radiance increase<\/li>\n<li><strong>Skin dark spots: <\/strong>Dark spots color decrease, Dark spots prevention, Dark spots removal, Dark circles under the eyes diminishment<\/li>\n<li><strong>Skin tanning <\/strong><\/li>\n<\/ul>\n<p><strong>Test: TYROSINASE ACTIVITY INHIBITION \/ ENHANCEMENT <\/strong><\/p>\n<p>Tyrosinase catalyzes the production of melanin in melanocytes. The inhibition of its activity leads to the skin lightening.<\/p>\n<p><em>In tubo test<\/em>, photometry.<\/p>\n<p><strong>Test: MENLANIN PRODUCTION EVALUATION<\/strong><\/p>\n<p>The increase on the melanin quantity gives to the skin a characteristic tanned color, and its decrease is beneficial in diminishing the appearance of the dark spots.<\/p>\n<p><em>In vitro<\/em> test on human normal melanocytes, spetro-photometric measurement.<\/p>\n<p>\n<\/div><\/div><\/div><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">2. CLAIM: Skin hydration<\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<ul>\n<li>Maintenance of the natural skin hydration level<\/li>\n<li>Increase of skin hydration<\/li>\n<\/ul>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>Test: INHIBITION OF THE HYALURONIDASE ACTIVITY<\/strong><\/p>\n<p>Hyaluronidase is an\u00a0endoglycosidase that breaks down hyaluronic acid. The inhibition of its activity protects hyaluronic acid from the degradation caused by the ageing processes.<\/p>\n<p><em>In tubo test<\/em>, photometry.<\/p>\n<p><strong>Test: EVALUATION OF THE HYALURONIC ACID PRODUCTION<\/strong><\/p>\n<p>Hyaluronic acid\u00a0is a\u00a0<a href=\"https:\/\/en.wikipedia.org\/wiki\/Glycosaminoglycan\">glycosaminoglycan<\/a>\u00a0which is a major component of skin, where it is involved in tissue repairing processes and skin hydration. It is known for its excellent water binding properties, since 1 molecule of the hyaluronic acid can bind about 250 water molecules. The amount of the hyaluronic acid in healthy skin decreases with age and after the UV exposure.<\/p>\n<p>ELISA test.<\/p>\n<p>\n<\/div><\/div><\/div><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">3. CLAIM: Skin microbiome<\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<ul>\n<li>Skin wellbeing maintenance<\/li>\n<li>Protection of the undisturbed skin microbiome<\/li>\n<li>Restoring of the skin microbiome balance<\/li>\n<\/ul>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>Test: MODULATION OF THE BACTERIAL PROPORTIONS IN THE SIMPLIFIED SKIN MICROBIOME MODEL<\/strong><\/p>\n<p>The maintenance or recovery of the skin microbiome balanced composition helps to maintain the skin wellbeing and health.<\/p>\n<p>Cocoulture of several bacterial strains characteristic for the human skin, measurement of the bacterial growth and density.<\/p>\n<p>\n<\/div><\/div><\/div><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">4. CLAIM: Skin elasticity<\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<ul>\n<li>Elastin protection<\/li>\n<li>Stimulation of the elastin production<\/li>\n<li>Maintenance \/ protection \/ recovery of the skin elasticity<\/li>\n<\/ul>\n<p><strong>Test: ELASTASE INHIBITION<\/strong><\/p>\n<p>Elastase is\u00a0a protease that breaks down elastin in the skin. The inhibition of its activity protects the elastin from the degradation caused by the ageing processes and the external factors, such as UV or air pollution.<\/p>\n<p><em>In tubo test<\/em>, photometry.<\/p>\n<p><strong>Test: ELASTIN PRODUCTION EVALUATION<\/strong><\/p>\n<p>This test evaluates the production of elastin by the normal human dermal fibroblasts.<\/p>\n<p>ELISA test or immunofluorescence.<\/p>\n<p>\n<\/div><\/div><\/div><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">5. CLAIM: Protection \/ recovery of the skin firmness, Anti-wrinkles efficacy <\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<ul>\n<li>Protection \/ recovery of the skin firmness<\/li>\n<li>Lifting effect<\/li>\n<li>Collagen protection<\/li>\n<li>Increase of the collagen in the skin<\/li>\n<\/ul>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>Test: COLLAGENASE ACTIVITY INHIBITION <\/strong><\/p>\n<p><strong>Collagenases<\/strong>\u00a0are\u00a0enzymes\u00a0that break the\u00a0peptide bonds\u00a0in\u00a0collagen.\u00a0The inhibition of the collagenase activity protects collagen from the degradation caused by the ageing processes and the external factors, such as UV or air pollution.<\/p>\n<p><em>In tubo test<\/em>, photometry.<\/p>\n<p><strong>Test: COLLAGEN PRODUCTION EVALUATION<\/strong><\/p>\n<p>This test evaluates the production of collagen I by the normal human dermal fibroblasts.<\/p>\n<p>ELISA test or immunofluorescence.<\/p>\n<p>\n<\/div><\/div><\/div><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">6. CLAIM: Protection from the ageing processes in the skin <\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<ul>\n<li>Protection of the extracellular matrix<\/li>\n<li>Protection of the skin structural proteins<\/li>\n<\/ul>\n<p><strong>Test: GENERAL OXIDATION PREVENTION<\/strong><\/p>\n<p>Oxidative stress in skin\u00a0plays a major role in the aging process. This is true for intrinsic aging and even more for extrinsic aging, driven to a large extent by oxidative stress caused by UV irradiation. During\u00a0oxidation, free radicals can\u00a0damage\u00a0the building blocks of healthy\u00a0skin. The inhibition of the oxidative stress protects the skin from the premature ageing and degradation.<\/p>\n<p><em>In tubo test<\/em>, photometry.<\/p>\n<p><strong>Test: PROTEIN GLYCOSYLATION PREVENTION<\/strong><\/p>\n<p>Skin\u00a0glycation\u00a0is\u00a0a process where excess sugar molecules bind to collagen and elastin fibers, leading to the formation of\u00a0advanced glycation end products\u00a0(AGEs). This process is one of the crucial mechanisms of the premature ageing.<\/p>\n<p><em>In tubo test<\/em>, photometry.<\/p>\n<p>\n<\/div><\/div><\/div><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">7. CLAIM: Reversing the skin ageing processes<\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<ul>\n<li>Skin renewal<\/li>\n<li>Induction of the skin natural repairing processes<\/li>\n<li>Reversing the ageing process of the skin cells<\/li>\n<\/ul>\n<p><strong>Test: EVALUATION OF THE GROWTH FACTORS MODULATION (EGF, VEGF, PDGF, FGF, TGF)<\/strong><\/p>\n<p>Growth factors are\u00a0naturally occurring proteins produced in various skin cells. These proteins are crucial in maintaining skin homeostasis to keep skin cells alive, healthy, and thriving.<\/p>\n<p>ELISA Test.<\/p>\n<p>\n<\/div><\/div><\/div><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">8. CLAIM: Anti-acne effect<\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<p><strong>Test: <em>CUTIBACTERIUM ACNES<\/em> (<em>PROPIONIBACTERIUM ACNES<\/em>) GROWTH MODULATION<\/strong><\/p>\n<p><em>Cutibacterium acnes\u00a0<\/em>is a member of the\u00a0skin\u00a0microbiota found predominantly in regions rich in sebaceous glands. It plays a role in the pathogenesis of acne lesions. The inhibition of its excessive growth helps to improve the acne symptoms.<\/p>\n<p>Bacterial culture, evaluation of the strain growth and density.<\/p>\n<p>\n<\/div><\/div><\/div><div class=\"bt_bb_accordion_item\"><div class=\"bt_bb_accordion_item_title\">9. CLAIM: Protection form the negative effect of the external factors (air pollution, solar radiation)<\/div><div class=\"bt_bb_accordion_item_content\"><div  class=\"bt_bb_text\" ><\/p>\n<ul>\n<li>Protection from the oxidative stress effect on the skin<\/li>\n<li>Protection from the negative effect of the air pollution and solar radiation on the skin<\/li>\n<li>Skin soothing effect<\/li>\n<li>Protection form inflammaging<\/li>\n<li>Protection of the cell processes for the natural ageing effects<\/li>\n<li>Protection of the hair follicle cells from the oxidative stress<\/li>\n<\/ul>\n<p><strong>Test: LIPID PEROXIDATION PREVENTION<\/strong><\/p>\n<p>Lipid peroxidation\u00a0occurs in skin-lipid bilayer membranes due to photo-oxidation. It has\u00a0a direct impact on the skin&#8217;s barrier shape. The inhibition of the lipid peroxidation\u00a0helps to decrease the negative effects of the solar radiation on the skin.<\/p>\n<p><em>In tubo test<\/em>, photometry.<\/p>\n<p><strong>Test: MODULATION OF THE INFLAMMATION<\/strong><\/p>\n<p>The inflammation processes in the skin are caused by both, the natural ageing process and the exogenous factors. They lead to the premature skin ageing (inflammaging) and the lesions appearance (ex. dark spots). The modulation of the biomarkers such as IL-1a, IL-2b, IL-6, IL-8, TNFa helps to control the skin inflammation.<\/p>\n<p>ELISA tests or immunofluorescence.<\/p>\n<p><strong>Test: REACTIVE OXYGEN SPECIES (ROS) EVALUATION<\/strong><\/p>\n<p>Oxidative stress in skin\u00a0plays a major role in the aging process. It is caused by both, intrinsic aging and the extrinsic one, driven by UV irradiation. During\u00a0oxidation, free radicals can\u00a0damage\u00a0the skin. The inhibition of the ROS activity helps to improve the cellular processes and skin overall shape.<\/p>\n<p>Fluorescence measurement.<\/p>\n<p>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><div class=\" bt_bb_row_inner\"   data-bt-override-class=\"{}\"><div class=\"bt_bb_row_inner_holder\"  ><\/div><\/div><div class=\" bt_bb_row_inner\"   data-bt-override-class=\"{}\"><div class=\"bt_bb_row_inner_holder\"  ><div  class=\"bt_bb_column_inner col-xxl-12 col-xl-12 col-xs-12 col-sm-12 col-md-12 col-lg-12 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal\" style=\"position: relative;\" data-width=\"12\" data-bt-override-class=\"{}\"><div class=\"bt_bb_column_inner_content\"><div class=\"bt_bb_column_inner_content_inner\"><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_30\" data-bt-override-class=\"null\"><\/div><header class=\"bt_bb_headline bt_bb_dash_top bt_bb_subheadline bt_bb_size_small bt_bb_align_left\" data-bt-override-class=\"null\"><h3 style=\"font-size:28px\"><span class=\"bt_bb_headline_content\"><span>IN VITRO SAFETY EVALUATION OF THE COSMETIC INGREDIENTS AND PRODUCTS:<\/span><\/span><\/h3><div class=\"bt_bb_headline_subheadline\"> <\/div><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_30\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_service bt_bb_color_scheme_20 bt_bb_style_filled bt_bb_size_small bt_bb_shape_square bt_bb_target_self bt_bb_align_inherit bt_bb_size_headline_inherit\" style=\"; --primary-color:#fff; --secondary-color:#6C76B2;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_small&quot;,&quot;def&quot;:&quot;small&quot;,&quot;xl&quot;:&quot;small&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><span  data-ico-fontawesome5regular=\"&#xf058;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_service_content\"><div class=\"bt_bb_service_content_text\">Determination of sunscreen UVA photoprotection in vitro, following ISO 24443:2021.<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_10\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_service bt_bb_color_scheme_20 bt_bb_style_filled bt_bb_size_small bt_bb_shape_square bt_bb_target_self bt_bb_align_inherit bt_bb_size_headline_inherit\" style=\"; --primary-color:#fff; --secondary-color:#6C76B2;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_small&quot;,&quot;def&quot;:&quot;small&quot;,&quot;xl&quot;:&quot;small&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><a href=\"https:\/\/www.chemotechnique.se\/patch-testing\/photopatch-testing-ppt---general-information-and-methodology-\/\"  target=\"_self\"   data-ico-fontawesome5regular=\"&#xf058;\" class=\"bt_bb_icon_holder\"><\/a><div class=\"bt_bb_service_content\"><div class=\"bt_bb_service_content_text\">In vitro phototoxicity test, following OECD 101 &amp; 432.<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_10\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_service bt_bb_color_scheme_20 bt_bb_style_filled bt_bb_size_small bt_bb_shape_square bt_bb_target_self bt_bb_align_inherit bt_bb_size_headline_inherit\" style=\"; --primary-color:#fff; --secondary-color:#6C76B2;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_small&quot;,&quot;def&quot;:&quot;small&quot;,&quot;xl&quot;:&quot;small&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19514927\/\"  target=\"_self\"   data-ico-fontawesome5regular=\"&#xf058;\" class=\"bt_bb_icon_holder\"><\/a><div class=\"bt_bb_service_content\"><div class=\"bt_bb_service_content_text\">Eye irritation in vitro test, following the OECD 491: Short Time Exposure In Vitro Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage.<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_10\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_service bt_bb_color_scheme_20 bt_bb_style_filled bt_bb_size_small bt_bb_shape_square bt_bb_target_self bt_bb_align_inherit bt_bb_size_headline_inherit\" style=\"; --primary-color:#fff; --secondary-color:#6C76B2;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_small&quot;,&quot;def&quot;:&quot;small&quot;,&quot;xl&quot;:&quot;small&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><span  data-ico-fontawesome5regular=\"&#xf058;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_service_content\"><div class=\"bt_bb_service_content_text\">Eye irritation in vitro test (HetCam).<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_10\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_service bt_bb_color_scheme_20 bt_bb_style_filled bt_bb_size_small bt_bb_shape_square bt_bb_target_self bt_bb_align_inherit bt_bb_size_headline_inherit\" style=\"; --primary-color:#fff; --secondary-color:#6C76B2;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_small&quot;,&quot;def&quot;:&quot;small&quot;,&quot;xl&quot;:&quot;small&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><span  data-ico-fontawesome5regular=\"&#xf058;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_service_content\"><div class=\"bt_bb_service_content_text\">In vitro skin sensitization (h-CLAT test), following OECD 442E.<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_10\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_service bt_bb_color_scheme_20 bt_bb_style_filled bt_bb_size_small bt_bb_shape_square bt_bb_target_self bt_bb_align_inherit bt_bb_size_headline_inherit\" style=\"; --primary-color:#fff; --secondary-color:#6C76B2;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_small&quot;,&quot;def&quot;:&quot;small&quot;,&quot;xl&quot;:&quot;small&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><span  data-ico-fontawesome5regular=\"&#xf058;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_service_content\"><div class=\"bt_bb_service_content_text\">In vitro skin irritation, following OECD 439.<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_10\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_service bt_bb_color_scheme_20 bt_bb_style_filled bt_bb_size_small bt_bb_shape_square bt_bb_target_self bt_bb_align_inherit bt_bb_size_headline_inherit\" style=\"; --primary-color:#fff; --secondary-color:#6C76B2;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_small&quot;,&quot;def&quot;:&quot;small&quot;,&quot;xl&quot;:&quot;small&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><span  data-ico-fontawesome5regular=\"&#xf058;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_service_content\"><div class=\"bt_bb_service_content_text\">Genotoxicity test (In Vitro Mammalian Cell Micronucleus Test), following OECD 487.<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_10\" data-bt-override-class=\"null\"><\/div><div class=\"bt_bb_service bt_bb_color_scheme_20 bt_bb_style_filled bt_bb_size_small bt_bb_shape_square bt_bb_target_self bt_bb_align_inherit bt_bb_size_headline_inherit\" style=\"; --primary-color:#fff; --secondary-color:#6C76B2;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_small&quot;,&quot;def&quot;:&quot;small&quot;,&quot;xl&quot;:&quot;small&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><span  data-ico-fontawesome5regular=\"&#xf058;\" class=\"bt_bb_icon_holder\"><\/span><div class=\"bt_bb_service_content\"><div class=\"bt_bb_service_content_text\">Mutagenicity test (bacterial reverse mutation test, Ames Test), following OECD 471.<\/div><\/div><\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_20 bt_bb_hidden_md bt_bb_hidden_lg\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><!-- cell_inner --><\/div><!-- cell --><\/div><!-- port --><\/section><section id=\"bt_bb_section69e49d5387778\" class=\"bt_bb_section bt_bb_color_scheme_1 bt_bb_layout_boxed_1200 bt_bb_vertical_align_top bt_bb_top_spacing_medium bt_bb_bottom_spacing_medium\" style=\"; --section-primary-color:#ffffff; --section-secondary-color:#000000;;background-color:rgb(108,118,178);\" data-bt-override-class=\"null\"><div class=\"bt_bb_port\"><div class=\"bt_bb_cell\"><div class=\"bt_bb_cell_inner\"><div class=\"bt_bb_row \"  data-bt-override-class=\"{}\"><div class=\"bt_bb_row_holder\" ><div  class=\"bt_bb_column col-xxl-8 col-xl-8 col-xs-12 col-sm-12 col-md-12 col-lg-8 bt_bb_vertical_align_top bt_bb_align_left bt_bb_padding_normal\" style=\"; --column-width:8;\" data-width=\"8\" data-bt-override-class=\"{&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_left&quot;,&quot;def&quot;:&quot;left&quot;,&quot;xl&quot;:&quot;left&quot;}}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><header class=\"bt_bb_headline bt_bb_color_scheme_1 bt_bb_dash_none bt_bb_superheadline bt_bb_size_medium bt_bb_align_inherit\" style=\"; --headline-primary-color:#ffffff; --headline-secondary-color:#000000;\" data-bt-override-class=\"null\"><h2><span class=\"bt_bb_headline_superheadline\"><span>CONTACT FORM<\/span><\/span><span class=\"bt_bb_headline_content\"><span>In vitro tests<\/span><\/span><\/h2><\/header><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_25\" data-bt-override-class=\"null\"><\/div><div  class=\"bt_bb_text\" ><\/p>\n<p>Questions? Need a customized offer?<br \/>\nUse our contact form. We will get back to you right away.<\/p>\n<p>\n<\/div><div class=\"bt_bb_separator bt_bb_border_style_none bt_bb_bottom_spacing_25\" data-bt-override-class=\"null\"><\/div><\/div><\/div><\/div><div  class=\"bt_bb_column col-xxl-4 col-xl-4 col-xs-12 col-sm-12 col-md-12 col-lg-4 bt_bb_vertical_align_middle bt_bb_align_left bt_bb_padding_normal\" style=\"; --column-width:4;\" data-width=\"4\" data-bt-override-class=\"{&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_left&quot;,&quot;def&quot;:&quot;left&quot;,&quot;xl&quot;:&quot;left&quot;}}\"><div class=\"bt_bb_column_content\"><div class=\"bt_bb_column_content_inner\"><div class=\"bt_bb_button bt_bb_color_scheme_6 bt_bb_icon_position_left bt_bb_style_outline bt_bb_size_normal bt_bb_width_inline bt_bb_shape_inherit bt_bb_target_self bt_bb_align_inherit\" style=\"; --primary-color:#ffffff; --secondary-color:#004787;\" data-bt-override-class=\"{&quot;bt_bb_size_&quot;:{&quot;current_class&quot;:&quot;bt_bb_size_normal&quot;,&quot;def&quot;:&quot;normal&quot;,&quot;xl&quot;:&quot;normal&quot;},&quot;bt_bb_align_&quot;:{&quot;current_class&quot;:&quot;bt_bb_align_inherit&quot;,&quot;def&quot;:&quot;inherit&quot;,&quot;xl&quot;:&quot;inherit&quot;}}\"><a href=\"\/kontakt\/\" target=\"_self\" class=\"bt_bb_link\" title=\"Contact us\"><span class=\"bt_bb_button_text\" >Contact us<\/span><\/a><\/div><\/div><\/div><\/div><\/div><\/div><\/div><!-- cell_inner --><\/div><!-- cell --><\/div><!-- port --><\/section><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":12468,"parent":5558,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-12461","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/pages\/12461","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/comments?post=12461"}],"version-history":[{"count":6,"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/pages\/12461\/revisions"}],"predecessor-version":[{"id":13280,"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/pages\/12461\/revisions\/13280"}],"up":[{"embeddable":true,"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/pages\/5558"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/media\/12468"}],"wp:attachment":[{"href":"https:\/\/www.gba-polska.pl\/en\/wp-json\/wp\/v2\/media?parent=12461"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}